ABSTRACT
In a modification of the glycerol jelly [GJ] reagent, five concentrations were prepared and tested for fixing the cover glass instantly while maintaining the high translucency of the fecal films. GJ reagent [7gm gelatin dissolved in 50ml boiling water added to 10ml glycerol] gave satisfactory results, especially with iodine and alkaline methylene blue mounts which stained the cytological structures of protozoa
Subject(s)
Clinical Laboratory Techniques/methods , Clinical Laboratory Techniques , Glycerol , Indicators and Reagents , Protozoan Infections/diagnosis , Eukaryota/isolation & purificationABSTRACT
Serodiagnosis of giardiasis by counterimmunoelectrophoresis assay [CIEP] and indirect immunofluorescence test [IFAT] were evaluated versus stool examination. Giardia Lamblia antibodies were detected in sera of 46.9% and 53.1% using CIEP and IFAT respectively out of 49 giardiasis-infected children [5-10 years] diagnosed microscopically, Among the control group [22 children] 13.6% and 18.2% were positive by CIEP and IFAT respectively. The findings of the study showed that we can not rely on serology to diagnose giardiasis specially in cases with low excretion rates
Subject(s)
Giardiasis/immunology , Serologic Tests/methods , Fluorescent Antibody Technique/methodsABSTRACT
Twenty nine sera of epileptic Egyptian recruits and 20 normal controls were examined to clarify the relation between toxoplasmosis and epilepsy using IgG and IgM indirect immunofluorescent test [IFAT]. 44.8% and 17.5% of the epileptic group showed IgG and IgM respectively against Toxoplasma. The control group showed higher positivity for IgG and IgM [65% and 30% respectively], which makes it hard to consider T. gondii as a cause of epilepsy. Statisticaly the relation as regards the percentage of positivity is insignificant as well [P>0.05]. However, high percentage of positivty of toxoplasmosis among epileptics should be taken into consideration
Subject(s)
Humans , Male , Epilepsy/etiology , Toxoplasmosis/complications , Toxoplasma/pathogenicityABSTRACT
Autopsies of fifty seven stray cats caught in Cairo, revealed intestinal parasites in 41 [71.9 per cent]. The infection rates were 40.35 per cent for Dipylidium caninum, 35.08 for Toxocara cati, 10.52 per cent for Diplopylidium sp., 8.77 per cent for Toxascaris sp., 7.01 per cent for both Heterophyes heterophyes and isospora felis, 5.26 per cent for Ancylostoma caninum and 3.5 per cent for Trichinella spiralis. Some cats had double infection and others triple infection. In addition to the general importance of these parasites to the health of the cat, most of them are transmissible to man causing different pathological manifestations. The epidemiological role of cat was discussed